Introduction

Minimum Information About an RNAi Experiment (MIARE) is a set of reporting guidelines which describes the minimum information that should be reported about an RNAi experiment to enable the unambiguous interpretation and reproduction of the results. MIARE forms part of a larger effort to develop RNAi data standards that include a data model, data exchange format, Controlled Vocabulary and supporting software tools.

Background

The MIARE reporting guidelines were initiated by members of the RNAi Global∞ in order to facilitate data sharing within the initiative. The guidelines were developed in part by a inter laboratory benchmarking study to identify minimal reporting parameters for high-throughput siRNA screens, and in part by workshops and discussions within the RNAi Global. The development of the MIARE standard is a collaborative effort driven by members of the RNAi community. We strongly encourage feedback on the draft reporting guidelines and the MIARE-Tab data exchange file. The aim is to develop these documents by consensus, so they can readily meet the needs of experimentalists who wish to share their high-throughput RNAi data in a standardised format, that allows meaningful comparisons across disparate datasets.

News

• MIARE reporting checklist, MIARE-Tab file and completed example - updated September 2009
• Zhang EE et al at the Genomics Institute of the Novartis Research Foundation deposit screening data from A genome-wide RNAi screen for modifiers of the circadian clock in human cells∞ into PubChem BioAssay∞ - September 2009
• Dharmacon,∞ in collaboration with NCBI,∞ upload a 'pilot' RNAi screening data set∞ into PubChem BioAssay,∞ successfully demonstrating the integration between MIARE-compliant data and PubChem∞ - March 2009
• MIARE talk by Dr. Anastasia Khvorova∞ at Keystone Symposia∞
• MIARE registered with MIBBI∞
• MIARE Mentioned Nature Biotechnology Editorial Standard Operating Procedures Nat Biotechnol. 2006 Nov;24(11):1299∞
• Chi's Discovery on Target Meeting∞ (Oct 23-26, 2006)
• MIARE mentioned in publication: Standards for Systems Biology Nat Rev Genet. 2006 Aug;7(8):593-605∞
• RNAi Global Initiative Members Meet to Establish Collaboration and Accelerate Biomedical Discovery Using Genome-Wide siRNA Library∞

Minimal Information About An RNAi Experiment (MIARE) – Proposed Reporting Guidelines

• Guidelines

Current Documents

• MIARE reporting checklist v0.7.6∞
• MIARE-Tab v0.7.6∞ - a simple Microsoft Excel-based data exchange format
• VZV Kinome Screen∞ - an example tab file (v0.7.6) completed with 'real-world' data
• Previous versions of documents

Mailing Lists

• miaredev-announce@lists.sourceforge.net∞ General MIARE Announcements
  You can browse∞ the list archive or subscribe/unsubscribe∞
  
• miare-objectmodel@lists.sourceforge.net∞ Discussion list for MIARE object model
  You can browse∞ the list archive or subscribe/unsubscribe∞

Forums

• MIARE Open Discussion∞
• MIARE Reporting guidelines∞
• MIARE Object Model∞

Links

• Minimum Information Resources
  • MIBBI, Minimum Information for Biological and Biomedical Investigations (MI Checklist Portal)
  • MIAME, gene expression microarray
    • MIAME-env, environmental genomics
    • MIAME-nut, nutrional genomics
    • MIAME-tox, toxogenomics
    • MIAME-Plant
  • MIAPE, proteomic experiments
    • MIAPE CC, reporting requirements for column chromatography
    • MIAPE CE, reporting requirements for capillary electrophoresis
    • MIAPE-MS, mass spectrometry
    • MIAPE SP, reporting requirements for sample preparation and handling
    • MIAPE-GE, gel electrophoresis
    • MIAPE GI, reporting requirements for gel informatics
    • MIMIX, Molecular Interaction experiment
  • MIACA, cell based assay
  • MIGS, genome sequence
  • MIFACE, flow cytometry
  • MISFISHIE, In Situ Hybridization and Immunohistochemistry Experiments
  • MIAPA, Phylogenetic Analysis
  • MIAO, ORF
  • MIAMET, METabolomics experiment
  • MIAFGE, Functional Genomics Experiment
  • MIRIAM, Minimum information requested in the annotation of biochemical models
  
  
• Data Standards
  • Omics A Journal of Integrative Biology, A Special Issue on Data Standards
  
  
• RNA Interference
  • RNAi Global, The RNAi Global Initiative
  • NCBI Gene Silencing Resource, NCBI database of RNAi reagents and experimental results generated using those reagents
  • The RNAi Web, web resource for RNAi
  
  
• Databases
  • PubChem, an NCBI database storing information from screens on the biological activities of small molecules

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