MIARE: Minimum Information About an RNAi Experiment : RNAi

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Most recent edit on 2007-02-27 09:29:38 by GraemeGrimes

Additions:
1) Presence of double stranded RNA (dsRNA) in cell.

Deletions:
1) Presence of double stranded RNA (dsRNA).



Edited on 2007-02-27 09:29:16 by GraemeGrimes

Additions:
Key feature of RNA Interference (RNAi)
  1. Presence of double stranded RNA (dsRNA).
  2. Binding of siRNA to complementary mRNA in the RNA-induced silencing complex (RISC).
  3. Degradation of the mRNA by argonaute, the catalytic component of the RISC.

    Deletions:
    RNA Interference (RNAi) can be classified by
  4. Presence of double stranded RNA (dsRNA)
  5. Binding of siRNA to complementary mRNA
  6. promotes cleavage of siRNA:mRNA complex



    Edited on 2007-02-27 09:25:09 by GraemeGrimes

    Additions:
    RNA Interference (RNAi) can be classified by
  7. Presence of double stranded RNA (dsRNA)
  8. Binding of siRNA to complementary mRNA
  9. promotes cleavage of siRNA:mRNA complex



    Edited on 2007-02-27 09:12:23 by GraemeGrimes

    Additions:
    - RNA interfererence (RNAi) describes the post-transcriptional silencing of gene expression that occurs in reponse to the introduction of double-stranded RNA into cells. Vanhecke D & Janitz M. Functional genomics using high-throughput RNA interference. Drug Discovery Today, Vol 10, No 3, pp 205-211.

    Deletions:
    - RNA interfererence (RNAi) describes the post-transcriptional silencing of gene expression that occurs in reponse to the introduction of double-stranded RNA into cells. Vanhecke D & Janitz M. Functional genomicsusing high-throughput RNA interference. Drug Discovery Today, Vol 10, No 3, pp 205-211.



    Edited on 2007-02-27 09:06:20 by GraemeGrimes

    Additions:
    - RNA interfererence (RNAi) describes the post-transcriptional silencing of gene expression that occurs in reponse to the introduction of double-stranded RNA into cells. Vanhecke D & Janitz M. Functional genomicsusing high-throughput RNA interference. Drug Discovery Today, Vol 10, No 3, pp 205-211.



    Edited on 2007-02-27 09:02:35 by GraemeGrimes

    Additions:
    - RNA interference refers to the process by which dsRNA molecules silence a target gene through the specific destruction of its mRNA. Echeverri C & Perrimon N. High-throughput RNAi screening in cultured cells: auser's guide. 2006, Nature Reviews, Vol 7; 373-383

    Deletions:
    - RNA interference refers to the process by which dsRNA molecules silence a target gene through the specific destruction of its mRNA. Echeverri C & Perrimon N. High-throughput RMAi screening in cultured cells: auser's guide. 2006, Nature Reviews, Vol 7; 373-383



    Edited on 2007-02-27 09:02:12 by GraemeGrimes

    Additions:
    - RNA interference refers to the process by which dsRNA molecules silence a target gene through the specific destruction of its mRNA. Echeverri C & Perrimon N. High-throughput RMAi screening in cultured cells: auser's guide. 2006, Nature Reviews, Vol 7; 373-383



    Edited on 2007-02-27 08:56:44 by GraemeGrimes

    Additions:
    - A conserved biological response to double-stranded RNA, known variously as RNA interference (RNAi) or post-transcriptional gene silencing, mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes. RNAi has been cultivated as a means to manipulate gene expression experimentally and to probe gene function on a whole-genome scale. Hannon G.J. RNA interference; Nature. 2002 Jul 11;418(6894):244-51.



    Edited on 2007-02-27 08:49:07 by GraemeGrimes

    Additions:
    - A gene silencing phenomenon whereby specific dsRNAs ( RNA, DOUBLE-STRANDED) trigger the degradation of homologous mRNA ( RNA, MESSENGER). The specific dsRNAs are processed into SMALL INTERFERING RNA (siRNA) which serves as a guide for cleavage of the homologous mRNA in the RNA-INDUCED SILENCING COMPLEX. DNA METHYLATION may also be triggered during this process. National Library of Medicine - Medical Subject Headings



    Edited on 2007-02-27 08:47:57 by GraemeGrimes

    No differences.


    Edited on 2007-02-27 08:47:49 by GraemeGrimes

    Additions:


    Deletions:
    -



    Edited on 2007-02-27 08:47:22 by GraemeGrimes

    Additions:
    -



    Edited on 2007-02-27 08:47:06 by GraemeGrimes

    Additions:
    - The process by which double-stranded RNAs silence cognate genes. Involves posttranscriptional gene inactivation ('silencing') both of transgenes or dsRNA introduced into a germline, and of the host gene(s) homologous to the transgenes or dsRNA. This silencing is triggered by the introduction of transgenes or double-stranded RNA (dsRNA), and occurs through a specific decrease in the level of mRNA of both host genes and transgenes. Gene Ontology
  10. RNA interference (RNAi) is a post-transcriptional gene silencing method reproducing a naturally occurring phenomena. RNAi is the process whereby double-stranded RNA (dsRNA) induces the sequence-specific degradation of homologous mRNA. RNAi or dsRNA-induced silencing phenomena are present in evolutionarily diverse organisms, e.g., nematodes, plants, fungi, and trypanosomes. The mechanisms by which RNAi works is initiated by a progressive cleavage of dsRNA into 21 to 23 nucleotide (nt) short interfering RNAs (siRNAs). These native siRNA duplexes are then incorporated into a protein complex called RNA-induced silencing complex (RISC). ATP-dependent unwinding of the siRNA duplex generates an active RISC complex. Guided by the antisense strand of siRNA, the active RISC complex recognizes and cleaves the corresponding mRNA. PSI Molecular Interaction Ontology 2.5



    Edited on 2007-02-27 08:42:52 by GraemeGrimes

    Additions:
    - Post-transcriptional gene silencing that regulates gene expression.- www.jco.org/cgi/glossarylookup

    Deletions:
    - -Post-transcriptional gene silencing that regulates gene expression.- www.jco.org/cgi/glossarylookup



    Edited on 2007-02-27 08:42:23 by GraemeGrimes

    Additions:
    -RNA interference. A natural cellular mechanism by which RNA is recognized as “foreign” due to its existence in a double-stranded form. This results in the degradation of the double-stranded RNA, along with single-stranded RNA having the same sequence.- www.sirna.com/glossary.html
  11. -Post-transcriptional gene silencing that regulates gene expression.- www.jco.org/cgi/glossarylookup
  12. RNA interference is a recently discovered functional tool. This is a phenomenon where an RNA introduced to a cell ultimately causes the degradation of the complementary cellular mRNA, and leads to the knockdown of gene activity.- www.invitrogen.com/content.cfm
  13. The use of long double stranded chains of ribonucleic acids to interfere and thus silence the expression of target genes- www.vastox.com/research/glossary.html
  14. RNA interference: A gene silencing phenomenon whereby double-stranded RNAs trigger the specific degradation of a homologous mRNA. The specific dsRNAs are processed into small interfering RNA (siRNA) which serves as a guide for cleavage of the homologous mRNA in the RNA- induced silencing complex (RISC). Useful as an application for specific suppression of an individual gene.- www.uark.edu/ua/ricecap/ricecapgloss.htm
  15. In molecular biology, RNA interference (RNAi) is a mechanism in which the presence of small fragments of double-stranded RNA (dsRNA) whose sequence matches a given gene interferes with the expression of that gene.- en.wikipedia.org/wiki/RNAi

    Deletions:
    -RNA interference. A natural cellular mechanism by which RNA is recognized as “foreign” due to its existence in a double-stranded form. This results in the degradation of the double-stranded RNA, along with single-stranded RNA having the same sequence.
  16. www.sirna.com/glossary.html
  17. A therapeutic approach for a range of genetic disorders where RNAi could be used to selectively turn off the disease gene in a mouse model. Turning off the disease gene in mice corrected neuroanatomical abnormalities and animals treated with RNAi had improved coordination. Even though RNAi can turn down bad genes, these genes are not completely silenced.
  18. www.hdsa.org/site/PageServer
  19. -Post-transcriptional gene silencing that regulates gene expression.
  20. www.jco.org/cgi/glossarylookup
  21. RNA interference is a recently discovered functional tool. This is a phenomenon where an RNA introduced to a cell ultimately causes the degradation of the complementary cellular mRNA, and leads to the knockdown of gene activity.
  22. www.invitrogen.com/content.cfm
  23. The use of long double stranded chains of ribonucleic acids to interfere and thus silence the expression of target genes
  24. www.vastox.com/research/glossary.html
  25. RNA interference: A gene silencing phenomenon whereby double-stranded RNAs trigger the specific degradation of a homologous mRNA. The specific dsRNAs are processed into small interfering RNA (siRNA) which serves as a guide for cleavage of the homologous mRNA in the RNA- induced silencing complex (RISC). Useful as an application for specific suppression of an individual gene.
  26. www.uark.edu/ua/ricecap/ricecapgloss.htm
  27. In molecular biology, RNA interference (RNAi) is a mechanism in which the presence of small fragments of double-stranded RNA (dsRNA) whose sequence matches a given gene interferes with the expression of that gene.
  28. en.wikipedia.org/wiki/RNAi



    Edited on 2007-02-27 08:41:06 by GraemeGrimes

    Additions:
    -RNA interference. A natural cellular mechanism by which RNA is recognized as “foreign” due to its existence in a double-stranded form. This results in the degradation of the double-stranded RNA, along with single-stranded RNA having the same sequence.
  29. www.sirna.com/glossary.html
  30. A therapeutic approach for a range of genetic disorders where RNAi could be used to selectively turn off the disease gene in a mouse model. Turning off the disease gene in mice corrected neuroanatomical abnormalities and animals treated with RNAi had improved coordination. Even though RNAi can turn down bad genes, these genes are not completely silenced.
  31. www.hdsa.org/site/PageServer
  32. -Post-transcriptional gene silencing that regulates gene expression.
  33. www.jco.org/cgi/glossarylookup
  34. RNA interference is a recently discovered functional tool. This is a phenomenon where an RNA introduced to a cell ultimately causes the degradation of the complementary cellular mRNA, and leads to the knockdown of gene activity.
  35. www.invitrogen.com/content.cfm
  36. The use of long double stranded chains of ribonucleic acids to interfere and thus silence the expression of target genes
  37. www.vastox.com/research/glossary.html
  38. RNA interference: A gene silencing phenomenon whereby double-stranded RNAs trigger the specific degradation of a homologous mRNA. The specific dsRNAs are processed into small interfering RNA (siRNA) which serves as a guide for cleavage of the homologous mRNA in the RNA- induced silencing complex (RISC). Useful as an application for specific suppression of an individual gene.
  39. www.uark.edu/ua/ricecap/ricecapgloss.htm
  40. In molecular biology, RNA interference (RNAi) is a mechanism in which the presence of small fragments of double-stranded RNA (dsRNA) whose sequence matches a given gene interferes with the expression of that gene.
  41. en.wikipedia.org/wiki/RNAi

    Deletions:
    -RNA interference. A natural cellular mechanism by which RNA is recognized as “foreign” due to its existence in a double-stranded form. This results in the degradation of the double-stranded RNA, along with single-stranded RNA having the same sequence.
www.sirna.com/glossary.html
-RNA inhibitor.
plan2005.cancer.gov/glossary.html
-A therapeutic approach for a range of genetic disorders where RNAi could be used to selectively turn off the disease gene in a mouse model. Turning off the disease gene in mice corrected neuroanatomical abnormalities and animals treated with RNAi had improved coordination. Even though RNAi can turn down bad genes, these genes are not completely silenced.
www.hdsa.org/site/PageServer
-Post-transcriptional gene silencing that regulates gene expression. (See siRNA [short-interfering RNA])
www.jco.org/cgi/glossarylookup
-RNA interference is a recently discovered functional tool. This is a phenomenon where an RNA introduced to a cell ultimately causes the degradation of the complementary cellular mRNA, and leads to the knockdown of gene activity.
www.invitrogen.com/content.cfm
-The use of long double stranded chains of ribonucleic acids to interfere and thus silence the expression of target genes
www.vastox.com/research/glossary.html
-RNA interference: A gene silencing phenomenon whereby double-stranded RNAs trigger the specific degradation of a homologous mRNA. The specific dsRNAs are processed into small interfering RNA (siRNA) which serves as a guide for cleavage of the homologous mRNA in the RNA- induced silencing complex (RISC). Useful as an application for specific suppression of an individual gene.
www.uark.edu/ua/ricecap/ricecapgloss.htm
-In molecular biology, RNA interference (RNAi) is a mechanism in which the presence of small fragments of double-stranded RNA (dsRNA) whose sequence matches a given gene interferes with the expression of that gene.
en.wikipedia.org/wiki/RNAi




Edited on 2007-02-27 08:38:59 by GraemeGrimes

Additions:
-RNA interference. A natural cellular mechanism by which RNA is recognized as “foreign” due to its existence in a double-stranded form. This results in the degradation of the double-stranded RNA, along with single-stranded RNA having the same sequence.
-RNA inhibitor.
-A therapeutic approach for a range of genetic disorders where RNAi could be used to selectively turn off the disease gene in a mouse model. Turning off the disease gene in mice corrected neuroanatomical abnormalities and animals treated with RNAi had improved coordination. Even though RNAi can turn down bad genes, these genes are not completely silenced.
-Post-transcriptional gene silencing that regulates gene expression. (See siRNA [short-interfering RNA])
-RNA interference is a recently discovered functional tool. This is a phenomenon where an RNA introduced to a cell ultimately causes the degradation of the complementary cellular mRNA, and leads to the knockdown of gene activity.
-The use of long double stranded chains of ribonucleic acids to interfere and thus silence the expression of target genes
-RNA interference: A gene silencing phenomenon whereby double-stranded RNAs trigger the specific degradation of a homologous mRNA. The specific dsRNAs are processed into small interfering RNA (siRNA) which serves as a guide for cleavage of the homologous mRNA in the RNA- induced silencing complex (RISC). Useful as an application for specific suppression of an individual gene.
-In molecular biology, RNA interference (RNAi) is a mechanism in which the presence of small fragments of double-stranded RNA (dsRNA) whose sequence matches a given gene interferes with the expression of that gene.


Deletions:
# RNA interference. A natural cellular mechanism by which RNA is recognized as “foreign” due to its existence in a double-stranded form. This results in the degradation of the double-stranded RNA, along with single-stranded RNA having the same sequence.
# RNA inhibitor.
# A therapeutic approach for a range of genetic disorders where RNAi could be used to selectively turn off the disease gene in a mouse model. Turning off the disease gene in mice corrected neuroanatomical abnormalities and animals treated with RNAi had improved coordination. Even though RNAi can turn down bad genes, these genes are not completely silenced.
# Post-transcriptional gene silencing that regulates gene expression. (See siRNA [short-interfering RNA])
# RNA interference is a recently discovered functional tool. This is a phenomenon where an RNA introduced to a cell ultimately causes the degradation of the complementary cellular mRNA, and leads to the knockdown of gene activity.
# The use of long double stranded chains of ribonucleic acids to interfere and thus silence the expression of target genes
# RNA interference: A gene silencing phenomenon whereby double-stranded RNAs trigger the specific degradation of a homologous mRNA. The specific dsRNAs are processed into small interfering RNA (siRNA) which serves as a guide for cleavage of the homologous mRNA in the RNA- induced silencing complex (RISC). Useful as an application for specific suppression of an individual gene.
# In molecular biology, RNA interference (RNAi) is a mechanism in which the presence of small fragments of double-stranded RNA (dsRNA) whose sequence matches a given gene interferes with the expression of that gene.




Oldest known version of this page was edited on 2007-02-27 08:37:41 by GraemeGrimes []
Page view:

Definitions


# RNA interference. A natural cellular mechanism by which RNA is recognized as “foreign” due to its existence in a double-stranded form. This results in the degradation of the double-stranded RNA, along with single-stranded RNA having the same sequence.
www.sirna.com/glossary.html

# RNA inhibitor.
plan2005.cancer.gov/glossary.html

# A therapeutic approach for a range of genetic disorders where RNAi could be used to selectively turn off the disease gene in a mouse model. Turning off the disease gene in mice corrected neuroanatomical abnormalities and animals treated with RNAi had improved coordination. Even though RNAi can turn down bad genes, these genes are not completely silenced.
www.hdsa.org/site/PageServer

# Post-transcriptional gene silencing that regulates gene expression. (See siRNA [short-interfering RNA])
www.jco.org/cgi/glossarylookup

# RNA interference is a recently discovered functional tool. This is a phenomenon where an RNA introduced to a cell ultimately causes the degradation of the complementary cellular mRNA, and leads to the knockdown of gene activity.
www.invitrogen.com/content.cfm

# The use of long double stranded chains of ribonucleic acids to interfere and thus silence the expression of target genes
www.vastox.com/research/glossary.html

# RNA interference: A gene silencing phenomenon whereby double-stranded RNAs trigger the specific degradation of a homologous mRNA. The specific dsRNAs are processed into small interfering RNA (siRNA) which serves as a guide for cleavage of the homologous mRNA in the RNA- induced silencing complex (RISC). Useful as an application for specific suppression of an individual gene.
www.uark.edu/ua/ricecap/ricecapgloss.htm

# In molecular biology, RNA interference (RNAi) is a mechanism in which the presence of small fragments of double-stranded RNA (dsRNA) whose sequence matches a given gene interferes with the expression of that gene.
en.wikipedia.org/wiki/RNAi
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